Advansta Inc.
Advansta Inc.
Blog Article

New blog articles detected.

  • Are you using the right loading control antibody?
    Loading control antibodies are a critical Western blotting tool that is often overlooked. They play an important role, ensuring that that you have loaded and transferred equivalent amounts of protein across your Western blot. If you are planning on comparing the protein level across samples, use a control to normalize your data for proper interpretation.... Read more » The post Are you using the ...
Advansta Inc.
Advansta Inc.
Blog Article

New blog articles detected.

  • Choosing the right primary
    Selecting a primary antibody will be one of the most important decisions that you will make when designing your next experiment. Choose wisely because an antibody with poor specificity will wreak havoc on your data, making interpretation nearly impossible. On the other hand, selecting an antibody with poor sensitivity will leave you scratching your head... Read more » The post Choosing the right ...
Advansta Inc.
Advansta Inc.
Blog Article

New blog articles detected.

  • Blocking Strategies for Westerns
    Everyone knows that the best offense is a good defense. So how do you defend your Western blot against background and non-specific binding? The best strategy ensures that your membrane is sufficiently blocked and your target’s epitopes are not masked. With so many different reagents to choose from it can be a daunting task to... Read more » The post Blocking Strategies for Westerns appeared first...
Advansta Inc.
Advansta Inc.
Blog Article

New blog articles detected.

  • How to Cyberstalk your Antibody
    By now, we have all honed our internet search skills to find information about pretty much anything and everything without leaving the comfort of our homes. We may not always be proud of our cyberstalking skills but we all know that knowledge is power and with so much information available at our fingertips, why aren’t... Read more » The post How to Cyberstalk your Antibody appeared first on Adva...
Advansta Inc.
Advansta Inc.
Blog Article

New blog articles detected.

  • The Ultimate SDS-PAGE Gel Making Tips and Tricks
    Prepare yourself for a true story. After graduation, a newly minted postdoc left our molecular biology lab to work in a clinical science lab in Germany. He developed a reputation in our lab for his SDS-PAGE gels, which gave the sharpest bands on Westerns. He passed along this protocol to some of the medical students... Read more » The post The Ultimate SDS-PAGE Gel Making Tips and Tricks appeare...
Advansta Inc.
Advansta Inc.
Blog Article

New blog articles detected.

  • How to get rid of your Western smile
    Sometimes your Western blot bands are not straight but  “smile” – the bands look more like crescent moons than straight lines. And, unlike your smile, a smiling Western is not a good thing. Band distortion makes it harder to determine the molecular weight of the target protein, complicates band shift detection and makes densitometry challenging.... Read more » The post How to get rid of your West...
  • Protein Transfer Method for All Seasons – Wet, Semidry, Dry
    Everybody has their favorite method of transferring proteins to the membrane and people reluctant to change them. There’s always “if it ain’t broken, don’t fix it” wisdom. But one day you will realize that your trusted tank irrevocably broke down. And on that black one day, it’s better to know what else is available and... Read more » The post Protein Transfer Method for All Seasons – Wet, Semidr...
  • My Fair Lady ELISABright™ and Logistic Regression
    Let’s say you have worked very hard and you have developed a perfect ELISA assay. Your plate is coated to perfection, non-specific binding is blocked and all samples have been properly diluted to fit your assay’s limits. Don’t let the wrong curve fit ruin your day! Choosing the Logistic Regression Model Whether you prefer direct... Read more » The post My Fair Lady ELISABright™ and Logistic Regre...
  • What is block doing in blots & ELISAs and how to improve it
    In many labs, blocks for Western Blot, Dot Blot, and ELISA are “one size fits all” – one buffer and one blocking agent for any antibody. If the combination doesn’t work, people would rather give up than try changing the mix. So why do we put a milky buffer on our membranes? Consider the block... Read more » The post What is block doing in blots & ELISAs and how to improve it appeared first on Adv...

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