Advansta Inc.
Advansta Inc.
Blog Post
  • The beginning of a new year is a great time to pause and reflect on the previous year’s accomplishments and shortcomings. The idea of making resolutions to improve oneself is a wonderful idea, unfortunately, many of us give up on our aspirations for self-improvement by February.     Here are some tips on how to... Read more » The post What’s Your New Year’s Resolution? appeared first on Advansta ...

Advansta Inc.
Advansta Inc.
Blog Post
  • With so many enhanced chemiluminescent substrates to choose from, it may seem overwhelming to select the substrate that will best suit your lab’s needs. Start by asking yourself the following questions:     Is the abundance of target protein in my sample low or high? Will I need to detect low and high abundance proteins... Read more » The post Which WesternBright™ Substrate is right for you? appe...

Advansta Inc.
Advansta Inc.
Blog Post
  • We’ve all been there before…. You worked hard all day and think that you have executed a brilliant experiment, only to realize that you generated data that looks like a hot mess. This is the worst feeling ever! The worst part is that there is literally nothing that you can do about it now. There... Read more » The post Top 5 Western Blotting Blunders appeared first on Advansta Inc..

Advansta Inc.
Advansta Inc.
Blog Post
  • Have you ever wondered why there are so many protein quantitation assays to choose from? I think I ask myself this question every time I need to use one! Accurate determination of protein concentration is critical for many experiments. Unfortunately, many of the detergents and reducing agents used to extract proteins are incompatible with the... Read more » The post How to Choose a Total Protein ...

Advansta Inc.
Advansta Inc.
Blog Post

ELISA Technical Tips

  • ELISA Technical Tips Enzyme-linked immunosorbent assay (ELISA or EIA) is a plate-based assay used to measure the concentration of an analyte in a sample. The analyte may be a peptide, protein, antibody or hormone. Depending on the approach you choose, an antibody or antigen is immobilized on the plate’s surface. The sample is added to... Read more » The post ELISA Technical Tips appeared first on...

Advansta Inc.
Advansta Inc.
Blog Post
  • Transitioning from Chemiluminescent to Fluorescent Western Blotting Do you regularly strip and re-probe your blots? Would you like a reliable way to quantify your bands? Consider adding a little color to your life with fluorescent Westerns. Fluorescent detection enables users to multiplex, which simplifies your workflow by eliminating stripping and re-probing. In addition, your data... Read more ...

Advansta Inc.
Advansta Inc.
Blog Post
  • Immunoprecipitation (IP) is a method used to enrich or purify your target protein from a complex mixture, such as a cell lysate, using a solid support. After IP you can analyze your samples by various techniques such as Western blot, ELISA or Mass Spectrometry. Some of the most critical factors to consider are the method... Read more » The post The Immunoprecipitation Guide appeared first on Adva...

Advansta Inc.
Advansta Inc.
Blog Post
  • Loading control antibodies are a critical Western blotting tool that is often overlooked. They play an important role, ensuring that that you have loaded and transferred equivalent amounts of protein across your Western blot. If you are planning on comparing the protein level across samples, use a control to normalize your data for proper interpretation.... Read more » The post Are you using the ...

Advansta Inc.
Advansta Inc.
Blog Post
  • Selecting a primary antibody will be one of the most important decisions that you will make when designing your next experiment. Choose wisely because an antibody with poor specificity will wreak havoc on your data, making interpretation nearly impossible. On the other hand, selecting an antibody with poor sensitivity will leave you scratching your head... Read more » The post Choosing the right ...

Advansta Inc.
Advansta Inc.
Blog Post
  • Everyone knows that the best offense is a good defense. So how do you defend your Western blot against background and non-specific binding? The best strategy ensures that your membrane is sufficiently blocked and your target’s epitopes are not masked. With so many different reagents to choose from it can be a daunting task to... Read more » The post Blocking Strategies for Westerns appeared first...

Advansta Inc.
Advansta Inc.
Blog Post
  • By now, we have all honed our internet search skills to find information about pretty much anything and everything without leaving the comfort of our homes. We may not always be proud of our cyberstalking skills but we all know that knowledge is power and with so much information available at our fingertips, why aren’t... Read more » The post How to Cyberstalk your Antibody appeared first on Adva...

Advansta Inc.
Advansta Inc.
Blog Post
  • Prepare yourself for a true story. After graduation, a newly minted postdoc left our molecular biology lab to work in a clinical science lab in Germany. He developed a reputation in our lab for his SDS-PAGE gels, which gave the sharpest bands on Westerns. He passed along this protocol to some of the medical students... Read more » The post The Ultimate SDS-PAGE Gel Making Tips and Tricks appeare...

Advansta Inc.
Advansta Inc.
Blog Post

New blog articles detected.

  • How to get rid of your Western smile

    Sometimes your Western blot bands are not straight but  “smile” – the bands look more like crescent moons than straight lines. And, unlike your smile, a smiling Western is not a good thing. Band distortion makes it harder to determine the molecular weight of the target protein, complicates band shift detection and makes densitometry challenging.... Read more » The post How to get rid of your West...

  • Protein Transfer Method for All Seasons – Wet, Semidry, Dry

    Everybody has their favorite method of transferring proteins to the membrane and people reluctant to change them. There’s always “if it ain’t broken, don’t fix it” wisdom. But one day you will realize that your trusted tank irrevocably broke down. And on that black one day, it’s better to know what else is available and... Read more » The post Protein Transfer Method for All Seasons – Wet, Semidr...

  • My Fair Lady ELISABright™ and Logistic Regression

    Let’s say you have worked very hard and you have developed a perfect ELISA assay. Your plate is coated to perfection, non-specific binding is blocked and all samples have been properly diluted to fit your assay’s limits. Don’t let the wrong curve fit ruin your day! Choosing the Logistic Regression Model Whether you prefer direct... Read more » The post My Fair Lady ELISABright™ and Logistic Regre...

  • What is block doing in blots & ELISAs and how to improve it

    In many labs, blocks for Western Blot, Dot Blot, and ELISA are “one size fits all” – one buffer and one blocking agent for any antibody. If the combination doesn’t work, people would rather give up than try changing the mix. So why do we put a milky buffer on our membranes? Consider the block... Read more » The post What is block doing in blots & ELISAs and how to improve it appeared first on Adv...

Out-Market Your Competitors?

Get complete competitive insights on over 2.2 million companies to drive your marketing strategy.

Create Free Account Log in

By signing up, you agree to the Terms of Service and Privacy Policy.

Out-Market Your Competitors

Get complete competitive insights on over 2.2 million companies to drive your marketing strategy.

Create Free Account

Already a user?  Log in

By signing up, you agree to the Terms of Service and Privacy Policy.