Oxford Nanopore Technologies Limited is a U.K.-based company which is developing and selling nanopore sequencing products (including the portable DNA sequencer, MinION) for the direct, electronic analysis of single molecules.

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Oxford Nanopore Technologies
Oxford Nanopore Technologies
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  • On 8th Feb 2018, Clive Brown, CTO of Oxford Nanopore, gave an update on Oxford Nanopore Technology. In this update he described the pathway to a sub $1000 genome using the PromethION device, introduced 'Flongle' - a smaller flow cell for smaller experiments and tests, outlined the pathway to very high consensus accuracy, and some other goodies.. watch for more.

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • The Gastrointestinal Bacteria Reference Unit at Public Health England (PHE) implemented whole genome sequencing (WGS) for outbreak detection and routine surveillance using the HiSeq Illumina platform in 2014. Recently, we explored ways of integrating Oxford Nanopore Minion Technology into our routine service for typing Shiga Toxin producing E. coli (STEC). A pilot study showed that outputs from th...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • The human/microbiome promises to be a new avenue to treat disease. However, we need model systems and research tools to understand the mechanistic drivers for the mammalian microbiome beyond current correlative metagenomics studies. Rutgers researchers are working with the MinION and mouse models to study how host genetics/exercise behaviour/ and exposure to evironmental toxins influences the host...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • Dr. Wei joined the Jackson Laboratory in 2016 as the Director of Genomic Technologies, where she actively engages the development and application of innovative sequencing based genomic technologies to explore genome function and make genome-based medical discoveries for solutions in treating human disease. Dr. Wei got her Ph.D. (Microbiology) from University of California at Davis. Prior to that s...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • Our core facility focuses on eukaryote functional genomics. Since 2010 we worked on Illumina high throughput sequencers providing RNA-seq and ChIP-seq results to our users. As estimation of transcript isoform abundance is a real challenge, we moved last year to Oxford Nanopore sequencing technology in order to get full length transcripts sequences. During this talk we will present the results we o...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • EpiBiome is working on bacteriophage therapeutics to meet the growing threat of antibiotic resistance. Bacteriophage are viruses that infect a narrow range of bacteria and can be used as a precisely targeted therapeutic. In order to effectively design phage cocktails, it is important to study both the phage’s and the bacterial host’s genomes. By incorporating the MinION into our current sequencing...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • Building upon the groundbreaking Read Until work of Matt Loose and his team, we have demonstrated an integrated software architecture, RUBRIC (Read Until with Basecall- and Reference-Informed Criteria), that represents a significant advancement and generalization of the Oxford Nanopore MinION’s nascent selective sequencing capability. Unlike the event trace (“squiggle”) pattern matching approach p...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • Microbial communities underpin all processes in the environment and have a direct impact on human health. However, despite their importance, only a tiny fraction of the millions of different microbes is known. This is mainly due to the immense difficulties of cultivating microbes from natural systems in the laboratory. The current standard approach to retrieve individual microbial genomes from co...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • Cancer genomes are characterized by changes in the genome on many different levels. In breast cancer, the HER2 amplicon is very important, but is not been well understood. It is known that the genes in the region of HER2 are increased in copy number, but the exact organization of the amplicon in cancer cells is not known. There is the potential for rearrangements between the genes and for differen...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • DNA was sequenced on the International Space Station (ISS) for the first time in August 2016 using a ground prepared library of genomic DNA from lambda bacteriophage, Escherichia coli, and Mus musculus. No decrease in sequencing performance was observed in space as compared to ground controls. Less than a year later, a sample preparation method including pipetting techniques, DNA amplification, an...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • High quality assemblies from larger complex genomes require reads that span repeat structures. Oxford Nanopore can generate ultra-long reads in excess of a megabase (Mb) to date and on average more than 50 kilobase (kb) if high molecular weight (HMW) DNA is used for library prep. These ultra-long reads enable assembly of complex nested transposable elements, large tandemly repeated gene clusters, ...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • Long read data has been shown to be key in producing highly contiguous assemblies for larger genomes, yet working with multiple large genomes using MinION has challenges. This presentation will discuss implementing the GridION in the Sanger Institute’s production sequencing facility, including the development of new LIMS, DNA quality assessment using FEMTO Pulse and data management and storage. I ...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • At the sequencing core facility of Toulouse (Get-PlaGe - France), we started to work with 3rd generation sequencers in 2015 with the installation of a PacBio RSII machine. Since almost three years, we have gained experience in library preparation for this type of sequencing, which is largely different from the library preparation for 2nd sequencing generation technologies (Illumina). In particular...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • Advancements in portable scientific instruments provide promising avenues to expedite field work in order to understand the diverse array of organisms that inhabit our planet. We tested the feasibility for in situ molecular analyses of endemic fauna using a portable laboratory fitting within a single backpack, in one of the world’s most imperiled biodiversity hotspots: the Ecuadorian Chocó rainfor...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • Hospital acquired pneumonia (HAP), respiratory infection developing more than 48 h after hospital admission, affects 1.5% (200k) of hospital inpatients per year in the UK and has a 25-50% attributable mortality rate associated with infection. Current culture based diagnosis has sub-optimal specificity and sensitivity and is too slow (more than 48 hrs) to impact on patient management. Rapid diagnos...

Oxford Nanopore Technologies
Oxford Nanopore Technologies
YouTube Video
  • PCR-based detection is still the most commonly used method for routine identification of viruses of clinical importance, and this approach is often complemented by Sanger sequencing for further genotype determination. Yet, point mutations and recombination events that occur frequently in the genomes of human viruses can potentially lead to false negative results when PCR-based techniques are uniqu...

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